In Vitro Biological Screening for Antimicrobial, DNA Cleavage Anti-Diabetic, Antioxidant, Anti-Inflammatory and Antihaemolytic of Some Metallocephalosporins

In vitroantimicrobial activity, DNA cleavage, anti-diabetic, antioxidant, anti-inflammotary and antihaemolytic of some synthesized Metallocephradines and Metallocefepimes were examined. In a broadspectrum of antibacterial and antifungal property, it was found that nickel (1:1) and copper (1:2) complexes were more active than cephradine against all tested bacteria except S.pyogenes and E.fecalis. Metallocefepimesare potentially good inhibitors of the fungi. It was found that chromium (2:1) and cobalt (2:1) cefepime complexes exerted significant activity towards A.flavus. They were eight times (MIC=0.49μg/ml) as active as the standard amphotericin (MIC=3.9μg/ml). It was found that all Metallocephradines acts as maltase, lactase sucrase, amylase and lipase inhibitors, except Cu-cephradine (1:2) and mixed metal (Fe-Ni)-cephradine complexes did not show inhibitory effect on maltase. On the other hand, all Metallocefepimes act as maltase, lactase, sucrose and amylase inhibitors. So, the presence of metals caused a significant inhibition in comparison with cefepime and control. The activity of the complexes in scavenging of free radical DPPH is fairly good but less than ascorbic acid (113.91%) as positive control, except Fe-cephradine complex in 2:1(M:L), which showed higher activity than ascorbic acid (124.90%).Also, Metallocephradines and Metallocefepimes were examined for in vitro anti-inflammatoryactivity and toxicity by HRBC. Metallocephradines showed significant antiinflammatory activity and safe, where the hemolysis index < 5% in a concentration dependent manner, except Ni (II)-cephradine complex in 1:1 (M:L) ratio and Cr (III)-cefepime complex in 2:1 (M:L) ratio exhibited toxicity (hemolysis index >5% ).